Human FATP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4659
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 466106
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human FATP2
Met1-Leu620
Accession # O14975
Met1-Leu620
Accession # O14975
Specificity
Detects human FATP2. Stains human FATP2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human FATP2 Antibody
Detection of FATP2 in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FATP2 Mono-clonal Anti-body (Catalog # MAB4659, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Anti-body (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human FATP2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FATP2
Fatty Acid Transport Protein 2 (FATP2), also known as SLC27A2 (solute carrier family 27, member 2), is a multipass transmembrane protein that participates in the transport and metabolism of long chain fatty acids. It has acyl-CoA synthase activity and contains an AMP-binding motif. FATP2 localizes to the endoplasmic reticulum and is predominantly expressed in the liver and kidney. Human FATP2 shares 82% aa sequence identity with mouse and rat FATP2.
Long Name
Fatty Acid Transport Protein 2
Alternate Names
ACSVL1, FACVL1, SLC27A2, THCA-CoA ligase, VLACS, VLCS
Gene Symbol
SLC27A2
UniProt
Additional FATP2 Products
Product Documents for Human FATP2 Antibody
Product Specific Notices for Human FATP2 Antibody
For research use only
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