Human Fc gamma RII/CD32 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1330

R&D Systems, part of Bio-Techne
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BAF1330
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AF1330
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Immunoprecipitation

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Fc gamma RII/CD32 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB
Ala46-Pro217
Accession # P31994

Specificity

Detects human Fc gamma RII/CD32 in Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant mouse CD32 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Fc gamma RII/CD32 Biotinylated Antibody

Fc? RII/CD32 antibody in Human PBMCs by Immunocytochemistry (ICC).

Fc gamma RII/CD32 in Human PBMCs.

Fc gamma RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with calcium ionomycin and PMA using Goat Anti-Human Fc gamma RII/CD32 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1330) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Fc gamma RII/CD32 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with calcium ionomycin and PMA

Western Blot

0.1 µg/mL
Sample: Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/CD32

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group, and the protein products are designated Fc gamma RIIA, B, and C (1). These receptors are glycoproteins of approximately 40 kDa having two extracellular Ig-like domains. The Fc gamma RII proteins share 94‑99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc gamma RIIA associates with FcR gamma, and delivers an activating signal upon ligand binding (3, 5). In contrast, Fc gamma RIIB delivers an inhibitory signal. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Its extracellular domain shares 99% amino acid identity with Fc gamma RIIB, but a portion of the cytoplasmic domain is closely related to Fc gamma RIIA. Fc gamma RII proteins are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin.

References

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Entrez Gene IDs

2212 (Human); 14130 (Mouse)

Gene Symbol

FCGR2A

UniProt

P31994

Additional Fc gamma RII/CD32 Products

Product Documents for Human Fc gamma RII/CD32 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Fc gamma RII/CD32 Biotinylated Antibody

For research use only

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