Human Fer Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7229

R&D Systems, part of Bio-Techne
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MAB7229
MAB7229-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 690318

View all Fer Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Fer
Asn93-Ala302
Accession # P16591

Specificity

Detects human Fer in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fer Antibody

Fer antibody in Human Liver by Immunohistochemistry (IHC-P).

Fer in Human Liver.

Fer was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Fer Monoclonal Antibody (Catalog # MAB7229) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Fer Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fer

FER [feline encephalitis virus (FES)-related kinase], also called Tyk3, is a widely expressed 822 amino acid (aa), 94 kDa intracellular non-receptor tyrosine kinase of the FES/FPS family. Human FER contains an FCH (microtubule/cytoskeleton interaction) domain (aa 1‑58), a coiled coil oligomerization region, an SH2 domain (aa 460‑550) and a kinase domain (aa 563‑816). FER is found both in the cytoplasm and the nucleus, and interacts with several transmembrane receptors, cytosolic proteins, and nuclear chromatin. Through its interactions and kinase activity, Tyk3/FER influences synapse formation, cytoskeletal rearrangement, cell survival and cell cycle progression. Within the region used as an immunogen, human Tyk3/FER shares 97% aa sequence identity with mouse Tyk3/FER. Potential human Tyk3/FER isoforms truncated at aa 308 and 478 have been described.

Long Name

Feline Encephalitis Virus-related Kinase

Alternate Names

Phosphoprotein NCP94, TYK3

Entrez Gene IDs

2241 (Human); 14158 (Mouse); 301737 (Rat)

Gene Symbol

FER

UniProt

P16591 (Human)

Additional Fer Products

Product Documents for Human Fer Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fer Antibody

For research use only

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