Western blot shows lysates of A549 human lung carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human FFAR3/GPR41 Monoclonal Antibody (Catalog # MAB10562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for FFAR3/GPR41 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of FFAR3/GPR41 in U937 Human Cell Line by Flow Cytometry

U937 human histiocytic lymphoma cell line was stained with Rabbit anti-human FFAR3/GPR41 monoclonal antibody (Catalog # MAB10562, open histogram) or isotype control antibody (MAB1050, open histogram) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human FFAR3/GPR41 MAb (Clone 2590G)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: U937 human histiocytic lymphoma cell line

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB10562 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FFAR3/GPR41

GPR41 and GPR42 are two closely related genes that are part of a cluster of four adjacent G protein-coupled receptors(GPR40, 41, 42, and 43). There are only six nucleotide and amino acid differences between GPR41 and GPR42. GPR41 is a receptor for short chain fatty acids. The rank order of potency for agonists of this receptor ispropionate = pentanoate= butyrate > acetate > formate. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation.

Long Name

Free Fatty Acid Receptor 3

Alternate Names

FFA3, FFA3R, GPR41

Entrez Gene IDs

2865 (Human); 233080 (Mouse); 365228 (Rat)

Gene Symbol

FFAR3

Additional FFAR3/GPR41 Products

Product Documents for Human FFAR3/GPR41 MAb (Clone 2590G)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FFAR3/GPR41 MAb (Clone 2590G)

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FFAR3/GPR41 MAb (Clone 2590G)

R&D Systems, part of Bio-Techne | Catalog # MAB10562

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FFAR3/GPR41 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FFAR3/GPR41 MAb (Clone 2590G)

Detection of Human FFAR3/GPR41 by Western Blot.