Human FGF-10 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF345

R&D Systems, part of Bio-Techne
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BAF345
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AF345
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all FGF-10 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-10
Cys37-Ser208
Accession # O15520

Specificity

Detects human FGF-10 in Western blots. In Western blots (under non-reducing conditions), approximately 30% cross-reactivity with recombinant human FGF-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human FGF-10 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF-10 (Catalog # 345-FG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-10

The FGFs are a growing family of heparin-binding growth factors that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share significant amino acid sequence homology. FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Human and mouse FGF-10 were subsequently cloned. The human FGF-10 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human FGF-10 shares approximately 92% and 95% amino acid sequence identity with mouse and rat FGF‑10, respectively. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

References

  1. Yamasaki, M. et al. (1996) J. Biol. Chem. 271:15918.
  2. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  3. Ohuchi, H. et al. (1997) Development 124:2235.
  4. Tagashira, S. et al. (1997) Gene 197:399.
  5. Bellusci, S. et al. (1997) Development 124:4867.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Entrez Gene IDs

2255 (Human); 14165 (Mouse); 25443 (Rat)

Gene Symbol

FGF10

UniProt

O15520

Additional FGF-10 Products

Product Documents for Human FGF-10 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human FGF-10 Biotinylated Antibody

For research use only

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