Detects human FGF-13 1B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -19, -20, -21, rhFGF basic, recombinant mouse FGF-8b, or -8c is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human FGF-13 1B Antibody

Detection of FGF‑13 in RPMI 8226 Human Cell Line by Flow Cytometry.

RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human FGF-13 1B Monoclonal Antibody (Catalog # MAB1909, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human FGF-13 1B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: RPMI 8226 human multiple myeloma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

1 µg/mL
Sample: Recombinant Human FGF-13 1B

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-13

FGF-13 (Fibroblast Growth Factor-13), also known as FGF homologous factor 2 (FHF-2), is a member of the FGF family. It is expressed in brain and skeletal muscle and has been implicated in nervous system development and function. The gene for FGF-13 has been localized in human chromosome X, in a region associated with Borjeson-Forssman-Lehmann syndrome, a syndromal X-linked mental retardation. Two alternately spliced isoforms that differ in their amino-terminal residues have been described.

Long Name

Fibroblast Growth Factor 13

Alternate Names

FGF13

Entrez Gene IDs

2258 (Human)

Gene Symbol

FGF13

UniProt

NP_378668

Additional FGF-13 Products

Product Documents for Human FGF-13 1B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FGF-13 1B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FGF-13 1B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1909

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FGF-13 Antibodies »

Product Specifications

Immunogen

Specificity