Human FGF-23 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2604

R&D Systems, part of Bio-Techne
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MAB2604
MAB2604-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 275802

View all FGF-23 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FGF-23
Tyr25-Ile251 (Arg179Gln)
Accession # Q9GZV9

Specificity

Detects human FGF-23 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -21, recombinant mouse FGF-8b, -8c, or -15.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human FGF-23 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human FGF-23 (Catalog # 2604-FG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-23

Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF family, within a subfamily that also includes FGF-19 and FGF-21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta-trefoil structure (1, 2). FGF-19 subfamily members are highly diffusible molecules owing to their poor ECM/heparin sulfate binding and plasma-stabilizing intramolecular folds (2-4). Mature human FGF-23 contains an atypical (very low affinity) heparin binding site (aa 134-162), a proteolytic cleavage site (Arg179-Ser180), and multiple O-linked glycosylation sites with Thr178 being of particular importance (4-7). O-linked glycosylation at Thr178 blocks the cleavage of FGF-23, thereby preventing loss of FGF-23 activity (7, 8). Mature human FGF-23 shows 72% aa identity to mouse FGF-23 and is active on mouse cells (6). FGF-23 exerts its effects through a ternary complex that includes Klotho and an FGF receptor (FGF R4 or the "c" isoforms of FGF R1 or FGF R3). Klotho has a restricted distribution that limits FGF-23 activity (9-11). FGF-23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels (12). FGF-23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by downregulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate (13).

References

  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
  3. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  4. Harmer, N.J. et al. (2004) Biochemistry 43:629.
  5. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
  6. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
  7. Frishberg, Y. et al. (2007) J. Bone Miner. Res. 22:235.
  8. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
  9. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  10. Urakawa, I. et al. (2006) Nature 444:770.
  11. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  12. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
  13. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.

Long Name

Fibroblast Growth Factor 23

Alternate Names

FGF23

Entrez Gene IDs

8074 (Human); 64654 (Mouse)

Gene Symbol

FGF23

UniProt

Q9GZV9

Additional FGF-23 Products

Product Documents for Human FGF-23 Antibody

Product Datasheets

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Product Specific Notices for Human FGF-23 Antibody

For research use only

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