Human FGF-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF235
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FGF-4
Ala31-Leu206
Accession # P08620
Ala31-Leu206
Accession # P08620
Specificity
Detects human FGF‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) FGF-6 is observed and less than 2% cross-reactivity with bovine FGF acidic, bovine FGF basic, rhFGF acidic, rhFGF basic, rh beta‑ECGF, rhFGF-5, rhFGF‑7, rhFGF‑8b, recombinant mouse (rm) FGF‑8b, rmFGF‑8c, and rhFGF‑10 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human FGF-4 Antibody
Cell Proliferation Induced by FGF‑4 and Neutralization by Human FGF‑4 Antibody.
Recombinant Human FGF-4 (Catalog # 235-F4) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235). The ND50 is typically 0.3-0.9 µg/mL.
FGF‑4 in Human Breast Cancer Tissue.
FGF-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human FGF-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human FGF-4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast and breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast and breast cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human FGF-4 (Catalog # 235-F4)
Sample: Recombinant Human FGF-4 (Catalog # 235-F4)
Neutralization
Measured by its ability to neutralize FGF‑4-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Rizzino, A. et al. (1988) Cancer Res. 48:4266]. The Neutralization Dose (ND50) is typically 0.3-0.9 µg/mL in the presence of 0.5 ng/mL Recombinant Human FGF‑4.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-4
FGF-4, the product of a developmentally regulated gene (hst-1), is a member of the FGF family that is efficiently secreted. The gene for FGF-4 (also known as FGFK or K-FGF for Kaposi sarcoma-associated FGF) was initially discovered as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors (including stomach and colon cancers, hepatocellular carcinomas, and Kaposi’s sarcomas). FGF-4 does not seem to be expressed in normal adult tissues. However, expression of the gene is spatially and temporally regulated during embryonic development. The murine homologue of human FGF-4 has been cloned and shown to be 82% homologous to the human protein at the amino acid sequence level. Human FGF-4 has been shown to exhibit cross species activity.
In vitro, FGF-4 is mitogenic for fibroblasts and endothelial cells. FGF-4 has been shown to be a potent angiogenesis promoter in vivo. FGF-4 has potent transforming potential apparently through an autocrine mechanism of action. FGF-4 plays a key role in limb development and has been identified as the molecular mediator of the activities of the apical ectodermal ridge that is required for directing the outgrowth and patterning of vertebrate limbs.
Long Name
Fibroblast Growth Factor 4
Alternate Names
FGF4, HBGF-4, HST-1, KFGF, KS3
Gene Symbol
FGF4
UniProt
Additional FGF-4 Products
Product Documents for Human FGF-4 Antibody
Product Specific Notices for Human FGF-4 Antibody
For research use only
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