I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: Human FGF basic/FGF2/bFGF Antibody
Brand: R&D Systems
SKU: MAB233
Rating: 4 (1 reviews)

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Human FGF basic/FGF2/bFGF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB233

(1)

Citations (7)

Product Datasheet / COA / SDS

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture, ELISA Microarray Development, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 10060

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF basic/FGF2/bFGF

Specificity

Recognizes human and bovine FGF basic/FGF2/bFGF. In capture ELISAs, shows approximately 0.2% cross-reactivity with bovine FGF acidic, and no cross-reactivity with recombinant human (rh) FGF acidic, rhFGF-4, rhFGF-6, or rhFGF-7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human FGF basic/FGF2/bFGF Antibody

Application

Recommended Usage

Human FGF basic/FGF2/bFGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human FGF basic/FGF2/bFGF Biotinylated Antibody (Catalog # BAM233)
Standard: Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein (Catalog # 233-FB)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB233 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF2, is a member of the FGF family of growth factors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for Human FGF basic/FGF2/bFGF Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody

For research use only

Citations
Reviews

Human FGF basic/FGF2/bFGF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB233

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Human FGF basic/FGF2/bFGF Antibody

Human FGF basic/FGF2/bFGF Sandwich Immunoassay

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Shipping

Stability & Storage

Background: FGF basic/FGF2/bFGF

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

Additional FGF basic/FGF2/bFGF Products

Product Documents for Human FGF basic/FGF2/bFGF Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody