Human Fibroblast Activation Protein alpha/FAP Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3715
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
Cell Selection, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha/FAP
Leu26-Asp760
Accession # Q12884
Leu26-Asp760
Accession # Q12884
Specificity
Detects human Fibroblast Activation Protein alpha/FAP in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images
Detection of Human Fibroblast Activation Protein alpha/FAP by Western Blot.
Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein a/FAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3715) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: WI‑38 human lung fibroblast cell line
Sample: WI‑38 human lung fibroblast cell line
Human Fibroblast Activation Protein alpha/FAP Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using BAF3715 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.
References
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Alternate Names
FAP, Seprase
Gene Symbol
FAP
UniProt
Additional Fibroblast Activation Protein alpha/FAP Products
- All Products for Fibroblast Activation Protein alpha/FAP
- Fibroblast Activation Protein alpha/FAP cDNA Clones
- Fibroblast Activation Protein alpha/FAP ELISA Kits
- Fibroblast Activation Protein alpha/FAP Lysates
- Fibroblast Activation Protein alpha/FAP Primary Antibodies
- Fibroblast Activation Protein alpha/FAP Proteins and Enzymes
- Fibroblast Activation Protein alpha/FAP Simple Plex
Product Specific Notices
For research use only
Loading...
Loading...
Loading...
Loading...