Human Fibronectin APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1918A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # P1H11
Product Specifications
Immunogen
Human plasma-derived Fibronectin
Specificity
Detects human Fibronectin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Fibronectin APC-conjugated Antibody
Detection of Fibronectin in WS-1 Human Cell Line by Flow Cytometry.
WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin APC-conjugated Monoclonal Antibody (Catalog # IC1918A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Fibronectin APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: WS‑1 human fetal skin fibroblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: WS‑1 human fetal skin fibroblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Human Fibronectin APC-conjugated Antibody
Product Specific Notices for Human Fibronectin APC-conjugated Antibody
For research use only
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