Human FLI1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6474
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FLI1
Ser13-Pro112
Accession # Q01543
Ser13-Pro112
Accession # Q01543
Specificity
Detects human FLI1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human FLI1 Antibody
Detection of Human FLI1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.5 µg/mL of Human FLI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FLI1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human FLI1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FLI1
FLI1 (Friend leukemia virus integration site 1; also ERGB) is a member of the ETS family of transcription factors. Although its predicted MW is 51 kDa. FLI1 is expressed by megakaryocytes, macrophages, B cells and embryonic endothelial cells. It is a transcriptional activator that regulates the genes for Tie-2, GpIIb, MPL and TGF-beta RII. Human FLI1 is 452 amino acids (aa) in length. It contains one PNT domain that is involved in heterotypic interactions (aa 112-198) and an ETS DNA‑binding domain (aa 281-361). There are two potential splice variants. One shows an alternative start site at Met34, while another contains a 10 aa substitution for aa 1‑76. There are a series of naturally occurring 62-68 kDa chimeric proteins (EWS/FLI1) in tumors that result from a chromosomal translocation involving the EWS and FLI1 genes. One creates a 499 aa chimera that includes aa 1-265 of EWS and 220-452 of FLI1. Over aa 13-112, human FLI1 shares 98% aa identity with mouse FLI1.
Long Name
Friend Leukemia Integration 1
Alternate Names
ERGB, SIC-1
Gene Symbol
FLI1
UniProt
Additional FLI1 Products
Product Documents for Human FLI1 Antibody
Product Specific Notices for Human FLI1 Antibody
For research use only
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