Human Flt-3 Ligand/FLT3L Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB308
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Papio cynocephalus (Yellow Baboon)
Applications
Validated:
Neutralization, Western Blot
Cited:
ELISA Development, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 40406
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt‑3 Ligand/FLT3L
Thr27-Pro185
Accession # AAA17999
Thr27-Pro185
Accession # AAA17999
Specificity
Detects human Flt-3 Ligand/FLT3L in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Flt-3 Ligand/FLT3L is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Flt-3 Ligand/FLT3L Antibody
Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody.
Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB308). The ND50 is typically 0.02-0.06 µg/mL.Applications for Human Flt-3 Ligand/FLT3L Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Flt‑3 Ligand/FLT3L (Catalog # 308-FK) under non-reducing conditions only.
Sample: Recombinant Human Flt‑3 Ligand/FLT3L (Catalog # 308-FK) under non-reducing conditions only.
Neutralization
Measured by its ability to neutralize Flt‑3 Ligand/FLT3L-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02‑0.06 µg/mL in the presence of 5 ng/mL Recombinant Human Flt‑3 Ligand/FLT3L.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Flt-3 Ligand/FLT3L
Long Name
fms-like Tyrosine Kinase 3 Ligand
Alternate Names
FLG3L, Flt3L, FLT3LG
Gene Symbol
FLT3LG
UniProt
Additional Flt-3 Ligand/FLT3L Products
Product Documents for Human Flt-3 Ligand/FLT3L Antibody
Product Specific Notices for Human Flt-3 Ligand/FLT3L Antibody
For research use only
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