Human Follistatin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB669

R&D Systems, part of Bio-Techne
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MAB669-100
MAB669-500
MAB669-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Canine, Goat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 85918

View all Follistatin Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Follistatin
Gly30-Asp329
Accession # P19883

Specificity

Detects human Follistatin in direct ELISAs and Western blots. It recognizes the 288 aa, 300 aa, and 315 aa isoforms of human Follistatin. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse Follistatin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Follistatin Antibody

Follistatin antibody in Human Pituitary by Immunohistochemistry (IHC-P).

Follistatin in Human Pituitary.

Follistatin was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Follistatin Monoclonal Antibody (Catalog # MAB669) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Follistatin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta and human pituitary

Western Blot

1 µg/mL
Sample: Recombinant Human Follistatin 300 aa 30-329 (Catalog # 669-FO)
under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB669 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin

Follistatin (FST) is a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa) protein with a 29 aa signal sequence, an N-terminal atypical TGF binding domain, three Follistatin domains that contain EGF-like and kazal-like motifs, and a highly acidic C-terminal tail. The first Follistatin domain (FS1) contains a heparin binding site, while FS1 and FS2 are most critical for activin binding and neutralization (3, 4). In addition to activin, Follistatin regulates bioavailability of many non-TGF-beta members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also regulates hematopoietic stem cell adhesion to fibronectin via FS2, and binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3 (3, 5, 6). Of three Follistatin isoforms, the full-length mature Follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative isoforms (5, 8, 9). The acidic tail is missing in the splice variant FST288 which shows the highest affinity for activins, while a partial tail exists in the proteolytically produced FST303, which shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa identity with mouse, rat, equine and ovine FST, 99% with porcine and 97% with bovine FST. Genetic deletion of Follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility (10).

References

  1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
  2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
  3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
  4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
  5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
  6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
  7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
  8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
  9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
  10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.

Alternate Names

FS, FST

Entrez Gene IDs

10468 (Human); 14313 (Mouse)

Gene Symbol

FST

UniProt

P19883

Additional Follistatin Products

Product Documents for Human Follistatin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Follistatin Antibody

For research use only

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