Human FosB/G0S3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2214
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FosB
Met1-Leu338
Accession # P53539
Met1-Leu338
Accession # P53539
Specificity
Detects human FosB.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human FosB/G0S3 Antibody
FosB/G0S3 in HeLa Human Cell Line.
FosB/G0S3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human FosB/G0S3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2214) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
FosB/G0S3 in Human Pancreas.
FosB/G0S3 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human FosB/G0S3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2214) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human FosB/G0S3 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast and human pancreas array
Sample: Immersion fixed paraffin-embedded sections of human breast and human pancreas array
Western Blot
0.1 µg/mL
Sample: Recombinant Human FosB/G0S3
Sample: Recombinant Human FosB/G0S3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FosB/G0S3
Long Name
FBJ Murine Osteosarcoma Viral Oncogene Homolog B
Alternate Names
G0S3
Gene Symbol
FOSB
UniProt
Additional FosB/G0S3 Products
Product Documents for Human FosB/G0S3 Antibody
Product Specific Notices for Human FosB/G0S3 Antibody
For research use only
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