Human Frk Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3766
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 393812
Product Specifications
Immunogen
E. coli-derived recombinant human Frk
Met1-Glu107
Accession # P42685
Met1-Glu107
Accession # P42685
Specificity
Detects human Frk in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human Frk Antibody
Frk in Human Breast Cancer Tissue.
Frk was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Rat Anti-Human Frk Monoclonal Antibody (Catalog # MAB3766) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in interlobular ducts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Frk Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frk
Fyn-related kinase (Frk) is a tyrosine kinase of the Src family. Also known as Rak, Gtk, and Iyk, Frk is predominantly expressed in epithelial tissues. Frk protein is 49% and 47% identical to Fyn and Csk, respectively. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.
Long Name
FYN-related Kinase
Alternate Names
Gtk, PTK5, Rak
Gene Symbol
FRK
UniProt
Additional Frk Products
Product Documents for Human Frk Antibody
Product Specific Notices for Human Frk Antibody
For research use only
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