Human FUBI/MNSF beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9036
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 861504
Product Specifications
Immunogen
E.coli-derived recombinant human FUBI/MNSF beta
Met1-Gly74
Accession # P35544
Met1-Gly74
Accession # P35544
Specificity
Detects human FUBI in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human FUBI/MNSF beta Antibody
Detection of Human FUBI/MNSF beta by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FUBI/MNSF beta at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FUBI/MNSF beta in HEK293 Human Cell Line.
FUBI/MNSF beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasmic puncta. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human FUBI/MNSF beta Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FUBI/MNSF beta
C-terminal gly-gly motif common to many ubiquitin-like proteins. FUBI/ MNSF beta conjugation to Bcl-G has been shown to regulate the ERK1/2-MAPK cascade in macrophage cell lines, and may be implicated in TLR4-mediated signal transduction. Conjugation to endophilin II regulates dectin-1-mediated phagocytosis and inflammatory responses, and may be implicated in TLR2 signaling pathway.
References
- Rossman,T.G. et al. (2003) Oncogene 22:1817.
- Nakamura, M. & Omura, S. (2008) Biosci. Biotech. Biochem. 72:1915.
- Nakamura, M. & Yamaguchi, S. (2006) J. Biol. Chem. 281:16861.
- Nakamura, M & Watanabe, N. (2010) Biochem, Biophys. Res. Comm. 15:275.
- Nakamura, M. et al. (2012) Mol. Cell Biochem. 364:39.
Alternate Names
40S Ribosomal Protein S30, asr1, FAU, Fub1, MNSFbeta, RPS30, S30
Gene Symbol
FAU
UniProt
Additional FUBI/MNSF beta Products
Product Documents for Human FUBI/MNSF beta Antibody
Product Specific Notices for Human FUBI/MNSF beta Antibody
For research use only
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