Human Fyn Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3574
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Fyn
Met1-Gly83
Accession # P06241
Met1-Gly83
Accession # P06241
Specificity
Detects human Fyn in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Fyn Antibody
Fyn in MOLT‑4 Human Cell Line.
Fyn was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human Fyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3574) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Fyn Antibody
Application
Recommended Usage
Immunocytochemistry
10-20 µg/mL
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line, Jurkat leukemic T cell line, and Ramos human Burkitt's lymphoma cell line
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line, Jurkat leukemic T cell line, and Ramos human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fyn
Fyn is a ubiquitously expressed member of the Src family of intracellular tyrosine kinases. Fyn activity is implicated in T cell receptor signaling, adhesion-mediated signaling, and regulation of brain function. Mice lacking fyn display various deficits, including impaired spatial memory, hypomyelination, and uncoordinated hippocampal structure, suggesting that Fyn is involved in multiple neural signaling pathways.
Long Name
FYN oncogene related to SRC, FGR, YES
Alternate Names
SLK, SYN
Entrez Gene IDs
2534 (Human)
Gene Symbol
FYN
UniProt
Additional Fyn Products
Product Documents for Human Fyn Antibody
Product Specific Notices for Human Fyn Antibody
For research use only
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