Human GABARAP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8574
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 853641
Product Summary for Human GABARAP Antibody
Immunogen
E. coli-derived recombinant human GABARAP
Accession # O95166
Accession # O95166
Specificity
Detects human GABARAP in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human GABARAP Antibody
Detection of Human and Mouse GABARAP by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 µM Chloroquine for 20 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human GABARAP Monoclonal Antibody (Catalog # MAB8574) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for GABARAP at approximately 14 & 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
GABARAP in A172 Human Cell Line.
GABARAP was detected in immersion fixed A172 human glioblastoma cell line using Rat Anti-Human GABARAP Monoclonal Antibody (Catalog # MAB8574) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus and cytoplasmic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GABARAP Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line
Sample: Immersion fixed A172 human glioblastoma cell line
Western Blot
2 µg/mL
Sample: A172 human glioblastoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line treated with Chloroquine
Sample: A172 human glioblastoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line treated with Chloroquine
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GABARAP
References
- Shpilka, T. et al. (2011) Genome Biol. 12:226.
- Wang, H. et al. (1999) Nature 397:69.
- Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
- Weidberg, H. et al. (2010) EMBO J. 29:1792.
- Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
Long Name
gamma-Aminobutyric Acid Type A Receptor Associated Protein
Alternate Names
Apg8p1, ATG8A, FLC3B, MM46
Gene Symbol
GABARAP
UniProt
Additional GABARAP Products
Product Documents for Human GABARAP Antibody
Product Specific Notices for Human GABARAP Antibody
For research use only
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