Human GAD1/GAD67 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2086

R&D Systems, part of Bio-Techne
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MAB2086
MAB2086-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 252910

View all GAD1/GAD67 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259

Specificity

Detects human GAD1/GAD67 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human GAD1/GAD67 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded section of human spinal cord

Western Blot

1 µg/mL
Sample: Recombinant Human GAD1/GAD67
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAD1/GAD67

GAD1, also known as GAD67, is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions and putative paracrine hormone in pancreatic islets. GAD occurs as two molecular forms, 65 kDa and 67 kDa, that are encoded by separate genes and share 65% amino acid identity. GAD1 is highly conserved and is expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary (1).

References

  1. Sheikh, S.N. et al. (1999) Neurochem. Int. 35:73.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Entrez Gene IDs

2571 (Human)

Gene Symbol

GAD1

UniProt

Q99259

Additional GAD1/GAD67 Products

Product Documents for Human GAD1/GAD67 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GAD1/GAD67 Antibody

For research use only

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