Human gamma-Synuclein Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5745
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human gamma-Synuclein
Asn47-Glu120
Accession # O76070
Asn47-Glu120
Accession # O76070
Specificity
Detects human gamma-Synuclein in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human beta-Synuclein is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human gamma-Synuclein Antibody
Detection of Human gamma-Synuclein by Western Blot.
Western blot shows lysates of human brain tissue. PVDF Membrane was probed with 1 µg/mL of Human gamma-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5745) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
gamma-Synuclein in Human Cerebellum.
gamma-Synuclein was detected in immersion fixed paraffin-embedded sections of human cerebellum using Human gamma-Synuclein Antigen Affinity Purified Polyclonal Antibody (Catalog # AF5745) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human gamma-Synuclein Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cerebellum
Sample: Immersion fixed paraffin-embedded sections of human cerebellum
Western Blot
1 µg/mL
Sample: Human brain tissue
Sample: Human brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: gamma-Synuclein
gamma-Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.
Alternate Names
BCSG1, PRSN, SNCG, Synoretin, Synuclein-gamma
Entrez Gene IDs
6623 (Human)
Gene Symbol
SNCG
UniProt
Additional gamma-Synuclein Products
Product Documents for Human gamma-Synuclein Antibody
Product Specific Notices for Human gamma-Synuclein Antibody
For research use only
Loading...
Loading...
Loading...
Loading...