Human GASP-1/WFIKKN2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB20701
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 288518
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKN2
Leu35-His576
Accession # Q8TEU8
Leu35-His576
Accession # Q8TEU8
Specificity
Detects human GASP‑1/WFIKKN2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human GASP-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GASP-1/WFIKKN2 Antibody
Detection of GASP-1/WFIKKN2 in Human Brain Cortex.
GASP-1/WFIKKN2 was detected in immersion fixed paraffin-embedded sections of Human Brain Cortex using Mouse Anti-Human GASP-1/WFIKKN2 Monoclonal Antibody (Catalog # MAB20701) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human GASP-1/WFIKKN2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Brain Cortex
Sample: Immersion fixed paraffin-embedded sections of Human Brain Cortex
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GASP-1/WFIKKN2
WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP‑1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP-2 is in the pancreas, liver, and thymus (4). GASP‑1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP‑1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.
References
- Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
- Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
- Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
- Trexler, M. et al. (2002) Biol. Chem. 383:223.
Long Name
Growth and Differentiation Factor-associated Serum Protein-1
Alternate Names
GASP1, WFIKKN2, WFIKKNRP
Gene Symbol
WFIKKN2
UniProt
Additional GASP-1/WFIKKN2 Products
Product Documents for Human GASP-1/WFIKKN2 Antibody
Product Specific Notices for Human GASP-1/WFIKKN2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...