Human GATA-1 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1779P
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 234732
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976
Met1-Ser413
Accession # P15976
Specificity
Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with recombinant human (rh) GATA-2 or rhGATA-6.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human GATA-1 PE-conjugated Antibody
Detection of GATA‑1 in MG‑63 Human Cell Line by Flow Cytometry.
MG-63 human osteosarcoma cell line was stained with Rat Anti-Human GATA-1 PE-conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human GATA-1 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MG‑63 human osteosarcoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: MG‑63 human osteosarcoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using IC1779P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: GATA-1
GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).
References
-
Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.
Alternate Names
GATA1
Gene Symbol
GATA1
UniProt
Additional GATA-1 Products
Product Documents for Human GATA-1 PE-conjugated Antibody
Product Specific Notices for Human GATA-1 PE-conjugated Antibody
For research use only
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