Human GATA-2 Biotinylated Antibody

Name: Human GATA-2 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2046

R&D Systems, part of Bio-Techne | Catalog # BAF2046

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

AF2046

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Phycoerythrin

IC2046P

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769

Specificity

Detects human GATA-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-5, and rhGATA-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human GATA-2 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human GATA-2

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-2

GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).

References

Tsai, S.F. et al. (1989) Nature 339:446.
Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Alternate Names

GATA2

Entrez Gene IDs

2624 (Human)

Gene Symbol