Human GATA-3 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC26051P
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Canine
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 291106
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Pro135-Ser258
Accession # P23771
Specificity
Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human GATA-1, -2, -4, -5, or -6.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GATA-3 PE-conjugated Antibody
Detection of GATA‑3 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 cells were stained with Mouse Anti-Human GATA-3 PE-conjugated Monoclonal Antibody (Catalog # IC26051P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human GATA-3 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using IC26051P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GATA-3
GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.
Additional GATA-3 Products
Product Documents for Human GATA-3 PE-conjugated Antibody
Product Specific Notices for Human GATA-3 PE-conjugated Antibody
For research use only
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