Human GATA-6 Biotinylated Antibody

Name: Human GATA-6 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1700

R&D Systems, part of Bio-Techne | Catalog # BAF1700

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Product Datasheet / COA / SDS

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Conjugate

Catalog #

Unconjugated

AF1700

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all GATA-6 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # Q92908-2

Specificity

Detects human GATA-6.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GATA-6 Biotinylated Antibody

GATA-6 in KATO-III Human Cell Line.

GATA-6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Human GATA-6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GATA-6 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed KATO-III human gastric carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human GATA-6

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-6

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Alternate Names

GATA6

Entrez Gene IDs

2627 (Human)

Gene Symbol