Human GDF-11/GDF-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB19582

R&D Systems, part of Bio-Techne
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MAB19582
MAB19582-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 743835

View all GDF-11/GDF-8 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GDF-11/BMP-11
Asn299-Ser407
Accession # O95390

Specificity

Detects human GDF-11/BMP-11 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with mouse GDF-8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human GDF-11/GDF-8 Antibody

Induction of Hemoglobin Expression by GDF‑11/BMP‑11 and Neutralization by anti-GDF‑11/GDF‑8 Antibody.

Induction of Hemoglobin Expression by GDF‑11/BMP‑11 and Neutralization by anti-GDF‑11/GDF‑8 Antibody.

Recombinant Human GDF-11/BMP-11 (Catalog # 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF-11/BMP-11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582). The ND50 is typically 0.04-0.2 µg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay.
Human GDF-11/BMP-11 Antibody in ELISA Standard Curve.

Human GDF‑11/BMP‑11 ELISA Standard Curve.

Recombinant Human GDF-11/BMP-11 protein was serially diluted 2-fold and captured by Mouse Anti-Human GDF-11/BMP-11 Monoclonal Antibody (Catalog # MAB19584) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human GDF-11/GDF-8 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human GDF‑11/BMP‑11 Monoclonal Antibody (Catalog # MAB19584).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human GDF-11/BMP-11 DuoSet ELISA Kit (Catalog # DY1958-05) for convenient development of a sandwich ELISA.

Neutralization

Measured by its ability to neutralize GDF‑11/BMP‑11 induction of hemoglobin expression in the K562 human chronic myelogenous leukemia cell line, Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND50) is typically 0.04-0.2 μg/mL in the presence of 25 ng/mL Recombinant Human GDF‑11/BMP‑11. This antibody also neutralizes GDF-8 in a similar K562 bioassay.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-11/GDF-8

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide processed into a 109 aa mature protein. Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. Mature GDF-11 and GDF-8 share 90% amino acid sequence identity. GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (2). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (3). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (4, 5).

References

  1. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
  2. Oh, S.P. et al. (2002) Genes & Dev. 16:274.
  3. Loffredo, F.S. et. al. (2013) Cell. 153.828.
  4. Katsimpardi, L. et. al. (2014) Science. 344.630.
  5. Sinha, M. et. al. (2014) Science. 344.649.

Long Name

Growth Differentiation Factor 11/8

Alternate Names

BMP11, BMP-11, bone morphogenetic protein 11, GDF-11, growth/differentiation factor 11, VHO

Entrez Gene IDs

10220 (Human)

Gene Symbol

GDF11

UniProt

O95390

Additional GDF-11/GDF-8 Products

Product Documents for Human GDF-11/GDF-8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human GDF-11/GDF-8 Antibody

For research use only

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