Human GLI-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3690
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2352B
Product Specifications
Immunogen
E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071
Met1-Glu479
Accession # P10071
Specificity
Detects human GLI-3 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human GLI-3 Antibody
Detection of GLI-3 in Human SW480 cell line.
GLI-3 was detected in immersion fixed SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI-3 Monoclonal Antibody (Catalog # MAB3690) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GLI-3 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed SW480 human fibroblast carcinoma cell line
Sample: Immersion fixed SW480 human fibroblast carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-3
GLI3 is a zinc finger protein. The GLI family of proteins are transcription factors and are mediators of Sonic hedgehog signaling. GLI3 is known to be a transcriptional repressor but also may have a positive transcription function. Mutations in the GLI3 gene are associated with many polydactyly diseases.
References
Rupert JM.,
Vogelstein B., Arheden K., Kinzler KW., "GLI3 Encodes a 190-kilodalton
Protein with Multiple Regions of GLI Similarity". Molecular and
Cellular Biology. 1990 Oct; 10(10): 5408-15.
Taipale J., Beachy PA., "The Hedgehog and Wnt Signaling
Pathways in Cancer". Nature. 2001 May;411(6835): 349-54.
Rash BG., Grove EA., "Patterning the Dorsal Telencephalon:
a Role for Sonic Hedgehog ?". The
Journal of Neuroscience. 2007 Oct; 27(43):
11595-603.
Long Name
GLI-Kruppel family member GLI3
Alternate Names
ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt
Gene Symbol
GLI3
UniProt
Additional GLI-3 Products
Product Documents for Human GLI-3 Antibody
Product Specific Notices for Human GLI-3 Antibody
For research use only
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