Human GluR7/GRIK3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB113281

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB113281-100
MAB113281-SP
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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2914A

View all GluR7/GRIK3 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human GluR7/GRIK3 
Gly249-Met1
Accession # Q13003

Specificity

Detects human GluR7/GRIK3 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human GluR7/GRIK3 Antibody

Detection of GluR7/GRIK3 in CHO cells by Flow Cytometry.

CHO cells transfected with hGRIK3 (filled histogram) vs irrelevant CHO transfectant cells (open histogram) were stained with Rabbit Anti-Human GluR7/GRIK3 Monoclonal Antibody (Catalog # MAB113281) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Applications for Human GluR7/GRIK3 Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: CHO cells transfected with hGRIK3 vs irrelevant CHO transfectant cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GluR7/GRIK3

GluR7 is a receptor for glutamate.  Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain. This gene can co-assemble with GRIK4 or GRIK5 to form heteromeric receptors. GluR7 is one of the five subtypes of kainate receptors.  Kainate receptors are widely expressed in the CNS and are involved in the regulation of transmitter release, synapse formation, and in the pathophysiology of brain diseases.  Genetic variants in the GluR7 gene are associated with schizophrenia, major depression, and bipolar disorder.

References

  1. Entrez Gene: GRIK3 glutamate receptor, ionotropic, kainate 3.
  2. Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.
  3. Begni S, Popoli M, Moraschi S, Bignotti S, Tura G.B, Gennarelli M., "Association Between the Ionotropic Glutamate Receptor Kainate 3 (GRI3) Ser310Ala Polymorphism and Schizophrenia". Mol Psychiatry. 2002; 7(4):416.

Long Name

Glutamate Receptor 7

Alternate Names

EAA5, GLR7, GluK3, GRIK3

Entrez Gene IDs

2899 (Human); 14807 (Mouse); 298521 (Rat)

Gene Symbol

GRIK3

UniProt

Q13003

Additional GluR7/GRIK3 Products

Product Documents for Human GluR7/GRIK3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GluR7/GRIK3 Antibody

For research use only

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