Human Glut1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB14181
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 202921
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571
Met1-Val492
Accession # AAA52571
Specificity
Detects human Glut1 in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human Glut1 Antibody
Detection of Human Glut1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Glut1 by Simple WesternTM.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human Glut1 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Western Blot
2 µg/mL
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glut1
Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑5). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.
References
- Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
- Meuckler, M. et al. 1985, Science 229:941.
- Jones, K.S. et al. 2006, J. Virol. 8291.
- Takenouchi, N. et al. 2007, J. Virol. 1506.
- Kinet, S. et al. 2007, Retrovirology 4:31.
Long Name
Glucose Transporter Type 1
Alternate Names
DYT17, DYT18, DYT9, EIG12, GLUT1DS, SLC2A1
Gene Symbol
SLC2A1
UniProt
Additional Glut1 Products
Product Documents for Human Glut1 Antibody
Product Specific Notices for Human Glut1 Antibody
For research use only
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