Recognizes human Glut2 expression on human Glut2‑transfected NS0 cells, but not on control transfectants. Was shown to detect Glut2 on the surface of the human intestinal cell line Caco2 (2) in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with human Glut1 or human Glut3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Glut2 PE-conjugated Antibody

Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut2 PE-conjugated Monoclonal Antibody (Catalog # FAB1414P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Glut2 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney, and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).

References

Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
Mahraoui, L. et al. (1994) J. Biochem. 298:629.

Long Name

Glucose Transporter Type 2

Alternate Names

SLC2A2

Entrez Gene IDs

6514 (Human); 20526 (Mouse)

Gene Symbol

SLC2A2

UniProt

P11168

Additional Glut2 Products

Product Documents for Human Glut2 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Glut2 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Glut2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1414P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications