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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 202017

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Glut3
Accession # P11169

Specificity

Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Glut3 Alexa Fluor® 488-conjugated Antibody

Detection of Glut3 antibody in NS0 Mouse Cell Line Transfected with Human Glut3 antibody by Flow Cytometry.

Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human Glut3 was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # FAB1415G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Glut3 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with human Glut3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut3

Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).

References

  1. Vannuci, S.J. et al. (1997) Glia 21:2.
  2. Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
  3. Illsley, N.P. (2000) Placenta 21:14.
  4. Mobasheri, A. (2002) Cell Biol. Int. 26:297.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Entrez Gene IDs

6515 (Human); 20527 (Mouse)

Gene Symbol

SLC2A3

UniProt

Additional Glut3 Products

Product Documents for Human Glut3 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Glut3 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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