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Human Glut4 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB86541G

R&D Systems, part of Bio-Techne
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FAB86541G-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 925932

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glut4
Met1-Asp509
Accession # P14672

Specificity

Detects human Glut4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Glut4 Alexa Fluor® 488-conjugated Antibody

Detection of Glut4 in HEK293 Human Cell Line Transfected with Human Glut4 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either human Glut4 (filled histogram) or irrelevant protein (open histogram) were stained with Mouse Anti-Human Glut4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB86541G). Cell were gated based on control antibody staining (IC0041G, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human Glut4 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Glut4
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut4

Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein and shares 65% aa identity with mouse Glut4.  It is an insulin-regulated glucose transporter.  Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer.  Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients.  Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.

Alternate Names

SLC2A4

Entrez Gene IDs

6517 (Human); 20528 (Mouse); 25139 (Rat)

Gene Symbol

SLC2A4

UniProt

Additional Glut4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Glut4 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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