HEK293 human embryonic kidney cell line transfected with either (A) human Glut4 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glut4 Monoclonal Antibody (Catalog # MAB86541) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Glut4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human Glut4 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut4

Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein and shares 65% aa identity with mouse Glut4. It is an insulin-regulated glucose transporter. Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer. Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients. Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.

Alternate Names

SLC2A4

Entrez Gene IDs

6517 (Human); 20528 (Mouse); 25139 (Rat)

Gene Symbol

SLC2A4

UniProt

P14672

Additional Glut4 Products

Product Documents for Human Glut4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Glut4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Glut4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB86541

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Glut4 Antibodies »

Product Summary for Human Glut4 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Glut4 Antibody

Detection of Glut4 in HEK293 Human Cell Line Transfected with Human Glut4 and eGFP by Flow Cytometry.