Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 195205

View all Glut5 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Glut5
Met1-Gln501
Accession # P22732

Specificity

Detects human Glut5. Stains human Glut5 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Glut5 Antibody

Detection of Glut5 antibody in NS0 Mouse Cell Line Transfected with Human Glut5 antibody by Flow Cytometry.

Detection of Glut5 in NS0 Mouse Cell Line Transfected with Human Glut5 by Flow Cytometry.

NS0 mouse myeloma cell line either transfected with human Glut5 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human Glut5 Monoclonal Antibody, followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Glut5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with human Glut5

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine and skeletal muscle

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut5

Glut5 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues. Glut5 is expressed at highest levels in small intestine and at lower levels in kidney, testis, skeletal muscle, and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis, and other tissues.

References

  1. Kayano, T. et al. (1990) J. Biol. Chem. 265:13276.
  2. Corpe, C.P. (2002) Biochem. Biophys. Acta. 1576:191.
  3. Sasaki, A. et al. (2003) Neurosci. Lett. 338:17.

Long Name

Glucose Transporter Type 5

Alternate Names

SLC2A5

Entrez Gene IDs

6518 (Human); 56485 (Mouse)

Gene Symbol

SLC2A5

UniProt

P22732

Additional Glut5 Products

Product Documents for Human Glut5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Glut5 Antibody

For research use only

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