Human Glutaredoxin 3/GLRX3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7560

R&D Systems, part of Bio-Techne
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MAB7560
MAB7560-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 791431

View all Glutaredoxin 3/GLRX3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glutaredoxin 3/GLRX3
Asn126-Lys294
Accession # O76003

Specificity

Detects human Glutaredoxin 3/GLRX3 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Glutaredoxin 3/GLRX3 Antibody

Detection of Human Glutaredoxin 3/ GLRX3 antibody by Western Blot.

Detection of Human Glutaredoxin 3/ GLRX3 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Glutaredoxin 3/GLRX3 Monoclonal Antibody (Catalog # MAB7560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Glutaredoxin 3/GLRX3 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC).

Glutaredoxin 3/GLRX3 in NIH-3T3 Mouse Cell Line.

Glutaredoxin 3/GLRX3 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human Glutaredoxin 3/ GLRX3 Monoclonal Antibody (Catalog # MAB7560) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Glutaredoxin 3/GLRX3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutaredoxin 3/GLRX3

GLRX3 (Glutaredoxin 3; also PICOT, PKC-theta-interacting protein, and Thioredoxin-like protein 2/TXNL2) is a 37-40 kDa member of the multidomain subgroup, monothiol glutaredoxin group of glutaredoxin/GTX proteins. It has widespread expression but is not found ubiquitously. Cells known to express cytosolic GLRX3 are principally epithelial in type, and include breast and adrenal epithelium, pancreatic exocrine gland epithelium, and proximal tubule renal epithelium. Cardiac muscle may also express GLRX3. By inference, it is suggested to be involved in cell proliferation and has also been associated with both PKC-theta regulation and MLP/muscle LIM protein-mediated cardiac contractility. Human GLRX3 is 335 amino acids (aa) in length. It contains an N-terminal thioredoxin-like domain (aa 2-117) that is followed by two PICOT homology domains (aa 144-236 and 237-335). The thioredoxin domain does not possess a typical disulfide, and it is suggested that this domain does not demonstrate redox activity. The PICOT domains will interact with intracellular proteins. GLRX3 exists as both a monomer and homodimer, with the homodimer incorporating two Fe/S clusters into their PICOT domains. These clusters serve as redox sensors within the cell. Over aa 126-294, human GLRX3 shares 98% aa sequence identity with mouse GLRX3.

Alternate Names

GLRX3, GLRX4, GRX3, GRX4, PICOT, TXNL2

Entrez Gene IDs

10539 (Human); 30926 (Mouse); 58815 (Rat)

Gene Symbol

GLRX3

UniProt

O76003 (Human)

Additional Glutaredoxin 3/GLRX3 Products

Product Documents for Human Glutaredoxin 3/GLRX3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Glutaredoxin 3/GLRX3 Antibody

For research use only

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