Human Glutathione S‑Transferase mu 1/
GS Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6894

R&D Systems, part of Bio-Techne
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AF6894
AF6894-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Glutathione S-Transferase mu 1/GSTM1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glutathione S‑Transferase mu 1/GS
Met1-Lys218
Accession # P09488

Specificity

Detects human Glutathione S‑Transferase mu 1/GS in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Glutathione S‑Transferase mu 1/GS Antibody

Detection of Human Glutathione S-Transferase mu 1/ GS antibody by Western Blot.

Detection of Human Glutathione S‑Transferase mu 1/ GS by Western Blot.

Western blot shows lysates of human kidney tissue, human adrenal gland tissue, human stomach tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Glutathione S-Transferase mu 1/GS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glutathione S-Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Glutathione S‑Transferase mu 1/GS Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Human kidney tissue, human adrenal gland tissue, human stomach tissue, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase mu 1/GSTM1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).

References

  1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
  2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
  3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
  4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
  5. Mo, Z. et al. (2009) Prostate. 69:662.
  6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
  7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.

Alternate Names

Glutathione STransferase mu 1, GST1, GSTM1, GTH4, GTM1

Entrez Gene IDs

2944 (Human)

Gene Symbol

GSTM1

UniProt

P09488

Additional Glutathione S-Transferase mu 1/GSTM1 Products

Product Documents for Human Glutathione S‑Transferase mu 1/GS Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Glutathione S‑Transferase mu 1/GS Antibody

For research use only

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