Human Glutathione S-Transferase pi 1/GSTP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6455

R&D Systems, part of Bio-Techne
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AF6455
AF6455-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Glutathione S-Transferase pi 1/GSTP1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1
Met1-Glu210
Accession # AAC51280

Specificity

Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Glutathione S-Transferase pi 1/GSTP1 Antibody

Glutathione S-Transferase pi 1/GSTP1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line.

Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Glutathione S-Transferase pi 1/GSTP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Glutathione S-Transferase pi 1/GSTP1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTP1 is a cytosolic protein that belongs to pi class of the GST superfamily. It is crystallized as a homodimer (1), but also exists in solution as an equilibrium mixture of monomer and dimer, depending on the protein concentration (2). Four genetic variants of GSTP1 with different enzymatic activities have been identified, which indicates the particular allelic form expressed in tissues could contribute to variation in catalytic efficiency and biological functions (3, 4). Human GSTP1 is present at elevated levels in many tumor cells, and has unique properties as a cancer marker (5). Genetic polymorphisms and expression patterns of GSTP1 have been associated with a variety of effects on human cancer, anti-cancer drug resistance, and asthma (6). In addition to its role as a drug-metabolizing enzyme, GSTP1 has ligand binding properties and regulates kinase signaling pathways through protein-protein interactions (7).

References

  1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.
  2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.
  3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.
  4. Hu, X. et al. (1998) Cancer Res. 58:5340.
  5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.
  6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.
  7. Adler, V. et al. (1999) EMBO J. 18:1321.

Alternate Names

DFN7, FAEES3, Glutathione STransferase pi 1, GSTP1

Entrez Gene IDs

2950 (Human)

Gene Symbol

GSTP1

UniProt

AAC51280

Additional Glutathione S-Transferase pi 1/GSTP1 Products

Product Documents for Human Glutathione S-Transferase pi 1/GSTP1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Glutathione S-Transferase pi 1/GSTP1 Antibody

For research use only

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