TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of Glypican 4 in Human BG01V Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Glypican 4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB91951).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: TF-1 Human Erythroleukemic Cell Line, and BG01V Human Embryonic Stem Cell

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 4

Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).

References

Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
Ussar, S. et al. (2012) Diabetes 61:2289.
Fico, A. et al. (2014) J. Neurosci. 34:8318.
Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
Strate, I. et al. (2015) Development 142:1767.

Alternate Names

GPC4, K-glypican

Entrez Gene IDs

2239 (Human); 14735 (Mouse); 317322 (Rat)

Gene Symbol

GPC4

UniProt

O75487

Additional Glypican 4 Products

Product Documents for Human Glypican 4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Glypican 4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Glypican 4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9195

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Glypican 4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Glypican 4 Antibody

Detection of Glypican 4 in Human TF-1 Cell Line by Flow Cytometry.