Human GM130/GOLGA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB81991
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2059C
Product Specifications
Immunogen
E. coli-derived recombinant human GM130/GOLGA2
Met258-Gln606
Accession # Q08379
Met258-Gln606
Accession # Q08379
Specificity
Detects human GM130/GOLGA2 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human GM130/GOLGA2 Antibody
Detection of Human GM130/GOLGA2 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, ZR-75 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human GM130/GOLGA2 Monoclonal Antibody (Catalog # MAB81991) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GM130/ GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GM130/GOLGA2 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and ZR‑75 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for GM130/GOLGA2 at approximately 141 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human GM130/GOLGA2 Monoclonal Antibody (Catalog # MAB81991) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human GM130/GOLGA2 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and ZR‑75 human breast cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and ZR‑75 human breast cancer cell line
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line, ZR‑75 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: MCF‑7 human breast cancer cell line, ZR‑75 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM130/GOLGA2
Long Name
Golgin A2
Alternate Names
GOLGA2, Golgin-95, SY11 Protein
Gene Symbol
GOLGA2
UniProt
Additional GM130/GOLGA2 Products
Product Documents for Human GM130/GOLGA2 Antibody
Product Specific Notices for Human GM130/GOLGA2 Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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