Human GPR108 Antibody

GPR108 in Human Prostate.

GPR108 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human GPR108 Monoclonal Antibody (Catalog # MAB11128) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human GPR108 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line (wild type) and HEK293T human embryonic kidney cell line transfected with human GPR108. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR108 Monoclonal Antibody (Catalog # MAB11128) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR108 at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.