HEK293 human embryonic kidney cell line transfected with (A) Human GRP12 or (B) irrelevant transfectant, and eGFP was stained with Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrants were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.

GPR12 in Human Brain.

GPR12 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human GPR12 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with human GRP12 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR12

GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.

Long Name

G Protein-coupled Receptor 12

Alternate Names

GPCR12, GPCR21

Entrez Gene IDs

2835 (Human); 14738 (Mouse); 80840 (Rat)

Gene Symbol

GPR12

UniProt

P47775

Additional GPR12 Products

Product Documents for Human GPR12 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR12 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR12 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6738

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPR12 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR12 Antibody

Detection of GPR12 in HEK293 Human Cell Line Transfected with human GRP12 and eGFP by Flow Cytometry.