Human GPR12 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6738P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 584940

View all GPR12 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPR12
Accession # P47775

Specificity

Detects human GPR12 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GPR12 PE-conjugated Antibody

Detection of GPR12 antibody in HEK293 Human Cell Line Transfected with Human GPR12 and eGFP antibody by Flow Cytometry.

Detection of GPR12 in HEK293 Human Cell Line Transfected with Human GPR12 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human GPR12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human GPR12 PE-conjugated Monoclonal Antibody (Catalog # FAB6738P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GPR12 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human GPR12 and EGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR12

GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.

Long Name

G Protein-coupled Receptor 12

Alternate Names

GPCR12, GPCR21

Entrez Gene IDs

2835 (Human); 14738 (Mouse); 80840 (Rat)

Gene Symbol

GPR12

UniProt

P47775

Additional GPR12 Products

Product Documents for Human GPR12 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR12 PE-conjugated Antibody

For research use only

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