Human GPR37 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB44501

R&D Systems, part of Bio-Techne
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MAB44501-100
MAB44501-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1036313

View all GPR37 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human GPR37
Leu28-Met265
Accession # O15354

Specificity

Detects human GPR37 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GPR37 Antibody

Detection of Human GPR37 by Western Blot.

Western blot shows lysates of human brain (motor cortex) and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR37 Monoclonal Antibody (Catalog # MAB44501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR37 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human GPR37 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human brain (motor cortex) and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR37

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 587 aa, 7-transmembrane receptor for the neuroprotective and glioprotective factor prosaposin  (1, 4).  It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus (2). It is a substrate of the E3 ubiquitin ligase, parkin, which is up-regulated during endoplasmic reticulum stress (3). In a juvenile form of Parkinson's disease, GPR37 accumulates, contributing to stress-induced neuronal cell death (2). The  extracelluar domains (aa 27-265) of human and mouse GPR37 share 68% aa identity.

References

  1. Marazziti, D. et al. (1997) Genomics, 45:68.
  2. Donohue, P.J. et al. (1998) Brain Res Mol Brain Res. 54:152.
  3. Omura, T. et al. (2006) J Neurochem. 99:1456.
  4. Meyer, R.C. et al. (2013) Proc. Natl. Acad. Sci. U. S. A. 110: 9529
 

Long Name

G Protein-coupled Receptor 37

Alternate Names

ETBR-LP-1, PAELR

Entrez Gene IDs

2861 (Human); 14763 (Mouse); 117549 (Rat)

Gene Symbol

GPR37

UniProt

O15354

Additional GPR37 Products

Product Documents for Human GPR37 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR37 Antibody

For research use only

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