NS0 cell line transfected with Human GPR64 and eGFP was stained with (A) Mouse Anti-Human GPR64 PE-conjugated Monoclonal Antibody (Catalog # FAB7977P) or (B) Mouse IgG2B PE-conjugated isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GPR64 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: NS0 Cell Line Transfected with Human GPR64 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR64

GPR64 (G-Protein coupled Receptor 64; also HE6) is a 110 kDa (predicted) member of the B class of GCPRs. Within this class GPR64 belongs to a Large N-termini family-B 7-transmembrane (LNB-7TM) subclass of receptors (also known as adhesion-GCPRs). GPR64 has restricted expression, being found in stereocilia cell membranes of epididymal caput epithelial cells and, to a limited extent, on osteoblasts. The function of GPR64 is somewhat unclear, but in the epididymis, it may be involved in fluid transport. Mature human GPR64 is a 980 amino acid (aa) 7-TM glycoprotein (SwissProt Q8IZP9). It contains an extended extracellular N-teminus (aa 38-627), seven consecutive TM segments (aa 628-878) and a C-terminal cytoplasmic tail (aa 879-1010). The extended extracellular region possesses a juxtamembrane GPS domain (aa 567-618) that serves as a proteolytic cleavage site. Enzymatic activity here generates a 180 kDa soluble form that stays associated with a 40 kDa membrane-embedded fragment. Notably, isolation of the membrane fragment gives rise to oligomers that run at > 250 kDa in SDS-PAGE. There are multiple splice variants. The one used for immunization to generate this antibody contains a deletion of aa 88-101 (RefSeq NP_001073328). Four other splice forms show single block deletions of aa 65-67, 51-66, 52-75, and 906-956, respectively. Three others possesses aa substitutions; a 20 aa block for aa 52-101, and a common 12 aa block that can substitute for either aa 68-101 or aa 52-101. Over aa 38-64 and 68-553 of RefSeq NP_001073328, human and mouse GPR64 share 69% aa sequence identity.

Long Name

G Protein-coupled Receptor 64

Alternate Names

EDDM6, HE6, TM7LN2

Entrez Gene IDs

10149 (Human); 237175 (Mouse); 266735 (Rat)

Gene Symbol

ADGRG2

UniProt

NP_001073328

Additional GPR64 Products

Product Documents for Human GPR64 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR64 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human GPR64 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7977P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPR64 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR64 PE-conjugated Antibody

Detection of GPR64 in NS0 Cell Line Transfected with Human GPR64 and eGFP by Flow Cytometry.