Human GPR81 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11134
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1048103
Product Specifications
Immunogen
Synthetic peptide corresponding to the N-term sequences of Human HCAR1/GPR81
Specificity
Detects human HCAR1/GPFR81 peptide in direct Elisa
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GPR81 Antibody
Detection of Human GPR81 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GPR81 Monoclonal Antibody (Catalog # MAB11134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR81 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human GPR81 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line
Sample: A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR81
Long Name
G Protein-coupled Receptor 81
Alternate Names
FKSG80, GPR104, HCA1, HCAR1, LACR1, TA-GPCR
Gene Symbol
HCAR1
Additional GPR81 Products
Product Documents for Human GPR81 Antibody
Product Specific Notices for Human GPR81 Antibody
For research use only
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