Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human GPR97 and eGFP. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human GPR97 Monoclonal Antibody (Catalog # MAB10097) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GPR97 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of GPR97 in HEK293 Human Cell Line Transfected with Human GPR97 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with human GPR97 and eGFP was stained with (A) Rabbit Anti-Human GPR97 Monoclonal Antibody (Catalog # MAB10097) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GPR97 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human GPR97 and eGFP

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human GPR97 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR97

Adhesion G protein-coupled receptor G3 (ADGRG3, GPR97 or PGR26) is an orphan GPCR receptor that in vitro regulates migration of lymphatic endothelial cells via the small GTPases RhoA and CDC42. GPR97 is a 549 aminoacids (aa) 7-transmembrane domain (7TM) receptor belonging to the secretin sub-family of GPCR. Human GPR97 is expressed in granulocytes and endothelial cells of the vasculature.

Long Name

G Protein-coupled Receptor 97

Alternate Names

PB99, PGR26

Entrez Gene IDs

222487 (Human); 54672 (Mouse); 291854 (Rat)

Gene Symbol

ADGRG3

UniProt

Q86Y34

Additional GPR97 Products

Product Documents for Human GPR97 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR97 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR97 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10097

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPR97 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR97 Antibody

Detection of Human GPR97 by Western Blot.