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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 481906

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPRC5A
Met1-Ser357
Accession # Q8NFJ5

Specificity

Stains human GPRC5A transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human GPRC5A Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GPRC5A

G-protein coupled receptor family C group 5 member A (GPRC5A), also known as Retinoic acid-induced gene 1 protein (RAIG-1), is a member of the G-protein coupled receptor 3 family. It has a molecular weight of approximately 35 kDa and contains seven transmembrane domains. Human GPRC5A shares 76% and 74% aa sequence identity with mouse and rat GPRC5A, respectively. It is expressed at high levels in fetal and adult lung and is also present in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at lower levels.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member A

Alternate Names

RAI3, RAIG1

Entrez Gene IDs

9052 (Human); 232431 (Mouse); 312790 (Rat)

Gene Symbol

GPRC5A

UniProt

Additional GPRC5A Products

Product Documents for Human GPRC5A Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GPRC5A Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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