Human GRAIL/RNF128 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6234
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GRAIL/RNF128
Val42-Pro161
Accession # Q8TEB7
Val42-Pro161
Accession # Q8TEB7
Specificity
Detects human GRAIL/RNF128 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GRAIL/RNF128 Antibody
Detection of Human GRAIL/RNF128 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human GRAIL/RNF128 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRAIL/RNF128 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human GRAIL/RNF128 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRAIL/RNF128
GRAIL (Gene related to anergy in lymphocytes protein; also RNF128) is a 45-47 kDa, type I transmembrane E3 ubiquitin ligase. It is expressed in the endosomes of select cell types such as early myeloid progenitors and lymphocytes, and mediates the ubiquitination of membrane proteins such as CD3, CD40L, and CD83, plus the tetraspanins CD81 and CD151. GRAIL apparently regulates the threshold for T cell activation, and as such, plays a key role in autoimmunity. In Treg cells, GRAIL promotes suppressive activity by restricting Th17 gene expression. Mature human GRAIL is 390 amino acids (aa) in length (aa 39-428). It possesses a 169 aa luminal region (aa 39-207) and a 190 aa cytoplasmic domain. The luminal region contains one PA (protease-associated) domain that mediates protein-protein interactions (aa 48-193), and the cytoplasmic segment shows one zinc-finger region (aa 277-318). There is one splice variant that shows a 134 aa substitution for aa 1-161. Over aa 42-161, human GRAIL shares 98% aa identity with mouse GRAIL.
Long Name
Gene Related to Anergy in Lymphocytes Protein/Ring Finger Protein 128
Alternate Names
RNF128
Gene Symbol
RNF128
UniProt
Additional GRAIL/RNF128 Products
Product Documents for Human GRAIL/RNF128 Antibody
Product Specific Notices for Human GRAIL/RNF128 Antibody
For research use only
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