Human Granzyme B Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC2906N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 351927
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144
Gly19-Tyr247
Accession # P10144
Specificity
Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Granzyme B Alexa Fluor® 700-conjugated Antibody
Detection of Granzyme B in NK92 cells by Flow Cytometry.
NK92 cells were stained with Mouse Anti-Human Granzyme B Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC2906N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human Granzyme B Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment was NK92 cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Granzyme B
Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).
References
- Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1549.
- Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
- Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
- Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
UniProt
Additional Granzyme B Products
Product Specific Notices for Human Granzyme B Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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